Fig. 2.

The effect of inhibition of PKC with Bis on CPI-17 phosphorylation. CPI-17 phosphorylation was significantly increased above basal values in response to histamine stimulation. Top: representative blots of CPI-17 phosphorylation in basal conditions, basal plus Bis, histamine, and histamine in the presence of Bis. Bottom: quantitative results of several CPI-17 blots. Histamine and stimulation significantly increased CPI-17 phosphorylation, which was significantly decreased in the presence of Bis. Values are means ± SE for at least 4 determinations. Significance from *basal in the absence of Bis, and **histamine in the absence of Bis: P ≤ 0.007. Statistics were determined by Student's t-test with Bonferroni correction.