Figure 5.

Gli-1 inhibitor zerumbone enhanced the radiosensitivity of human glioblastoma cells. (a) U87MG cell radiation survival curves. D0 values (radiation dose with 37% survival) of U87MG cells for control and zerumbone were 4.6 Gy and 4.9 Gy, respectively. (b) U373MG cell radiation survival curves. D0 values (radiation dose with 37% survival) of U373MG cells for control and zerumbone were 4.0 Gy and 3.6 Gy, respectively. Cells were plated for colony formation assay after radiation. After 10–14 days, the dishes were stained with crystal violet, and colonies containing ≥50 cells were counted. Data from three separate experiments were expressed as mean ± standard error of mean