Figure 1.

Increase in BAG3 expression in HK2 cells exposed to FGF-2. (a) HK2 cells were treated with the indicated dose of FGF-2 for 24 h, BAG3 mRNA was measured using real-time RT-PCR. (b) HK2 cells were treated with the indicated dose of FGF-2 for 24 h, mRNA levels of mesenchymal markers α-SMA and VIM were analyzed using real-time RT-PCR. (c) HK2 cells were treated with the indicated dose of FGF-2 for 24 h, Western blot analysis was performed using the indicated antibodies. (d) HK2 cells were pretreated with vehicle, 5 µM of U0126, 10 µM of LY294002, or 5 µM of rottlerin for 30 min, then treated with 10 ng/mL of FGF-2 for additional 24 h, real-time RT-PCR was performed to investigate BAG3 mRNA expression. (e) HK2 cells were pretreated with vehicle, 5 µM of U0126, 10 µM of LY294002, or 5 µM of rottlerin for 30 min, then treated with 10 ng/mL of FGF-2 for additional 24 h, BAG3 protein expression was measured using Western blot analysis. *P < .01. N.S.: not significant; BAG3: Bcl-2-associated athanogene 3; FGF-2: fibroblast growth factor-2