Figure 3.

Biochemical phenotype of the freshly isolated human GPR125⁺ spermatogonia by MACS. (a) Double immunostaining revealed the co-expression of GFRA1 and PLZF in the freshly isolated human GPR125-positive spermatogonia. (b) RT-PCR showed the transcripts of GPR125, GFRA1, RET, PLZF, UCHL1, MAGEA4, SYCP3, SYCP1, PRM1, and TNP1 in the isolated human GPR125-positive spermatogonia and human testicular cells. ACTB was used as a loading control of total RNA, while PCR without primers served as a negative control. (A color version of this figure is available in the online journal.)