Figure 6.

Gene and protein characteristics of human GPR125-positive spermatogonia when cultured for 1 month. (a) Double immunostaining revealed the co-expression of GFRA1 and PLZF in human GPR125-positive spermatogonia after 30 days of culture. (b) RT-PCR showed the expression of GPR125, GFRA1, RET, PLZF, UCHL1, MAGEA4, SYCP3, SYCP1, PRM1, and TNP1 in human GPR125-positive spermatogonia when cultured for 30 days and human testicular cells. ACTB was used as a loading control of total RNA. (A color version of this figure is available in the online journal.)