. 2015 Jun 18;240(8):1112–1122. doi: 10.1177/1535370215590822

Table 1.

Primers of numerous genes used for RT-PCR

Genes	Primer sequence		Product size (bp)	Tm (℃)
GPR125	Forward	TACCCTTTGGACTTGGTT	193	49
GPR125	Reverse	CTCCATTCGTCCCATTAG	193	49
GFRA1	Forward	CCAAAGGGAACAACTGCCTG	121	50
GFRA1	Reverse	CGGTTGCAGACATCGTTGGA	121	50
PLZF	Forward	CGGTTCCTGGATAGTTTGC	317	50
PLZF	Reverse	GGGTGGTCGCCTGTATGT	317	50
RET	Forward	CTCGTTCATCGGGACTTG	126	50
RET	Reverse	ACCCTGGCTCCTCTTCAC	126	50
UCHL1	Forward	CCAATGTCGGGTAGATGA	244	55
UCHL1	Reverse	CAAAGTCCCTCCCACAGA	244	55
MAGEA4	Forward	CCGAGTCCCTGAAGATG	155	50
MAGEA4	Reverse	CAGGACGATTATCAGAAGG	155	50
SYCP1	Forward	AACTACTGTCTGCAGCTTGG	100	60
SYCP1	Reverse	CATCTCTTCCAGCTCACTTGAT	100	60
SYCP3	Forward	TGCAGGAGTAGTTGAAGATATG	110	60
SYCP3	Reverse	GCAAGAAGAGCCTTGTTAATG	110	60
PRM1	Forward	ATAGCACATCCACCAAACTCC	106	60
PRM1	Reverse	AGGCGGCATTGTTCCTTAG	106	60
TNP1	Forward	CGACCAGCCGCAAATTAAAG	105	60
TNP1	Reverse	CAGGTTGCCCTTACGGTATT	105	60
ACTB	Forward	CGCACCACTGGCATTGTCAT	253	55
ACTB	Reverse	TTCTCCTTGATGTCACGCAC	253	55