Figure 2.

Cytotoxicity effects of long chain fatty acid esters of Q3G on HepG2 cells. The figure describes the percentage of LDH release from HepG2 cells after treatment with the test compounds. Cells (5 × 10³ cells per well; 96-well plate) were treated with 100 µM of the test compounds for 6 h (a) and 24 h (b). After treatment, the cells were centrifuged and the percentage of LDH release from the cells was determined by LDH assay. All long chain fatty acid esters of Q3G except stearic acid ester showed over 80% cell death via LDH release within 6 h of treatment. Mean separation between groups was conducted using Tukey's test (n = 6). Significance level was taken at P value < 0.05