Figure 1.

Vaspin treatment enhanced lipid accumulation during adipocyte differentiation in 3T3-L1 cells. (a) 3T3L1 preadipocytes were treated with various concentrations of vaspin during differentiation, and cell viability was assessed using an MTT assay. (b) Oil Red O was extracted from the cells using isopropyl alcohol, and the absorbance was measured at 490 nm. (c) The cells were incubated with or without vaspin for six days. The cells were fixed with formalin and then stained with Oil Red O (200 × magnification). *P < 0.05 compared with control. (A color version of this figure is available in the online journal.)