Figure 2.

Effects of various concentrations of vaspin on the expression of adipocyte-specific markers. The cells were incubated with or without vaspin for six days. (a, b, and c) The mRNA levels of the C/EBP and PPAR-γ transcription factors and the expression of the adipogenic marker gene FABP4 were analyzed using RT-PCR analysis on day 6 of differentiation. (d and e) The protein expression levels of PPAR-γ and FABP4 were assessed using western blot analysis on day 6 of differentiation. *P < 0.05 compared with the control group; **P < 0.01 compared with the control group