Figure 3.

Effects of HIF-1α gene silencing on HIF-1α protein and mRNA levels. The HUVECs treated with DMOG only for 4 h as control group (D), Control), or pretreated with 50 nM mismatched siRNA (mmsiRNA), 50 nM, or 100 nM HIF-1α siRNA for 72 h in serum-free media before exposed to DMOG for 4 h. a Changes in the total protein levels of HIF-1α. b HIF-1α mRNA levels measured by real-time RT-PCR. Data were obtained from three independent experiments, and each experiment contains triplicate samples for each treatment. Values are means ± SEM. *Significantly different from control group control group (p < 0.05)