Figure 3.

Exposure of the human HASMCs with Ang II caused AT2R but not AT1R mRNA and proteins level to increase significantly. Human ASMCs were incubated in the absence or presence of 1 µM Ang II for 48 h. The cell lysates were then collected. Levels of AT1R and AT2R protein in cell lysates were respectively measured by RT-PCR and Western blot. (a, c, e, and g): Bar graphs corresponding to mean results of three independent experiments normalized to their corresponding loading controls. (b and f): Examples of qPCR for AT1R and AT2R mRNA. (d and h): Representative Western blots for evaluation of AT1R and AT2R protein in cell lysates. GAPDH protein is shown as the loading control. Values are expressed as mean ± SEM of three individual experiments. The cells without Ang II treatment were used as control. ^**P < 0.01 compared with control cells in the absence of Ang II stimulation