Figure 2.

miR-125a targets BMPR2 in human pulmonary artery endothelial cells. (a) Addition of anti-miRs directed against miR-125a significantly decreased the levels of miR-125a in cultured HPAEC (n = 6). (b) As shown by qPCR analysis inhibition of miR-125a slightly upregulated the mRNA levels of BMPR2 in HPAEC when compared to scrambled transfected cells (n = 6). (c) Protein levels of BMPR2 were found significantly increased in anti-miR-125a transfected HPAEC (n = 6). A representative Western blot is shown. (d) Transfection of HPASMC with anti-miR-125a significantly repressed the levels of miR-125a (n = 6), but (e) did not change the expression of BMPR2 on the mRNA (n = 6) as well as (f) on the protein level (n = 4). A representative Western blot is provided. Statistical analysis by unpaired Student's t-test (*p < 0.05, **p < 0.01, ***p < 0.001)