Figure 5.

Hypoxia-induced expression of miR-125a correlates with downregulation of BMPR2 and CDKN1A in vivo. (A) The expression of BMPR2 and CDKN1A was measured by qPCR in lung tissue samples obtained from mice that developed PH after chronic exposure to hypoxia (same samples as in Figure 1(b)). When compared to normoxic samples, a significant decrease of the mRNA expression of BMPR2 and CDKN1A was observed (n = 8). (b) A significant inverse correlation was found when the expression of miR-125a was compared to the levels of BMPR2 and CDKN1A in lungs of mice with PH (n = 8). Statistical analysis by unpaired Student's t-test, Pearson's correlation, and Mann–Whitney test (*p < 0.05, ***p < 0.001)