Figure 7.

Blockade of ERK1/2 phosphorylation with kinase deficient ERK1(K71R) reversed FLCM-induced hepatic differentiation of hUCMSCs. hUCMSCs were transfected with control vector or pFLAG-CMV-ERK1(K71R) and cultured in DMEM or FLCM for different times. A: Expression of P-ERK and T-ERK in hUCMSCs transfected with control vector or pFLAG-CMV-ERK1(K71R) by Western blotting analysis at day 2 during FLCM treatment; B and C: Expression of hepatocyte-specific genes evaluated by RT-PCR(B) and immunocytochemistry analysis(C). (A color version of this figure is available in the online journal.)