Table 1.
Primers for RT-PCR and real-time PCR
| Genes | Primer Sequence (5′–3′) | Annealing Temperature(℃) | Product size (bp) |
|---|---|---|---|
| AFP | For: TGCAGCCAAAGTGAAGAGGGAAGA | 63 | 216 |
| Rev: CATAGCGAGCAGCCCAAAGAAGAA | |||
| TDO | For: ACCTCCGTGCTTCTCAGACA | 55 | 493 |
| Rev: GGAAGCCTGATGCTGGAGAT | |||
| CK18 | For: GGAGGCATCCAGAACGAGAA | 58 | 376 |
| Rev: CCAGCTGCAGTCGTGTGATA | |||
| Nanog | For: ATGCCTCACACGGAGACTG | 60 | 369 |
| Rev: CTGCGTCACACCATTGCTA | |||
| CYP1A1 | For: ACCATGACCAGAAGCTATGGGT | 60 | 215 |
| Rev: TTAACACCTTGTCGATAGCACCA | |||
| ALB | For: TTGCCAAGC TGCTGATAAGG | 58.5 | 238 |
| Rev: ATGGCAGCA TTCCGTGTG | |||
| ABCG2 | For: CGGCTTGCAACAACTATGAC | 60 | 537 |
| Rev: ATCCTGCTTGGAAGGCTCTA | |||
| OCT4 | For: TATACACAGGCCGATGTGG | 60 | 397 |
| Rev: GTGCATAGTCGCTGCTTGA | |||
| CD44 | For: TCACAGGTGGAAGAAGAGAC | 57 | 447 |
| Rev: CATTGCCACTGTTGATCACT | |||
| CYP3A4 | For:CCAAGCTATGCTCTTTCACCG | 60 | 324 |
| Rev:TCAGGCTCCACTTACGGTGC | |||
| GAPDH | For: GGATTTGGTCGTATTGGG | 55 | 205 |
| Rev: GGAAGATGGTGATGGGATT |