Figure 2.

EZH2 expression level in EZH2-siRNA transfected SW620 cells. A: EZH2 mRNA expression of EZH2-siRNA transfected SW620 cells detected by RT-PCR. Forty-eight hours later of EZH2-siRNA transfection, RT-PCR was performed to detect EZH2 mRNA expression. B: EZH2 protein expression of EZH2-siRNA transfected SW620 cells detected by Western blot. Forty-eight hours later of EZH2-siRNA transfection of SW620 cells, protein expression of EZH2 was determined by western blot assay. a: EZH2-siRNA group; b: scrambled-siRNA group; c: non-siRNA group; d to f: represented corresponding internal reference. *, compared with scrambled-siRNA group and non-siRNA group, P < 0.05. The results shown were representative of three independent experiments