Figure 3.

BCL-2 is the target of miR-204. (a) Sequence alignment of miR-204 and 3′-UTR of BCL-2 using mirco-RNA.org. (b) AGE1.HN and PC12 cells were transfected with miR-204 mimic or miR-204 inhibitor or controls under normal oxygen conditions. The expression of BCL-2 was detected with qRT-PCR. (c) Relative luciferase activity in cells transfected with miR-204 mimics (or miR-204 inhibitor) and luciferase expression vector of BCL-2 under normal oxygen conditions. (d) AGE1.HN and PC12 cells were transfected with miR-204 mimic or miR-204 inhibitor or controls under normal oxygen conditions. The protein expression of BCL-2 was detected with Western blotting. (e) AGE1.HN and PC12 cells were transfected with miR-204 inhibitor or controls and then exposed to hypoxia. The protein expression of BCL-2 was detected with Western blotting. The data are the mean ± SD. **P < 0.01 vs. control; ^#P < 0.05 vs. inhibitor control. (A color version of this figure is available in the online journal.)