Figure 4.

Hypoxia increases the expression of miR-204 and reduces the expression of BCL-2 via HIF1-α pathway. (a) AGE1.HN and PC12 cells were treated with 1 µmol/L and 10 µmol/L YC-1 for 6 h and then exposed to hypoxia for 24 h or transfected with siHIF-1α. The protein expression of HIF1α was detected with Western blotting. (b) AGE1.HN and PC12 cells were treated with 1 µmol/L and 10 µmol/L YC-1 for 6 h and then exposed to hypoxia for 24 h or transfected with siHIF-2α. The protein expression of HIF-2α was detected with Western blotting. (c) The mRNA level of miR-204 was detected in cells transfected with YC-1 or siHIF-1α or siHIF-2α and exposed to hypoxia. (d) The mRNA level of BCL-2 was detected in cells transfected with YC-1 or siHIF-1α or siHIF-2α and exposed to hypoxia. (e) The protein level of BCL-2 was detected in AGE1.HN and PC12 cells transfected with YC-1 and siHIF-1α and exposed to hypoxia. (f) The percentage of apoptotic cells was detected by flow cytometry in AGE1.HN and PC12 cells transfected with YC-1 or siHIF-1α. The data are the mean ± SD. **P < 0.01 vs. control; ^#P < 0.05 vs. hypoxia