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Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) reduction increases the production and secretion of transforming growth factor β1 (TGF-β1). MRC-5 cells were transfected with PTEN siRNA or negative control (NC) siRNA for 48 h in serum-free medium. (a) Western blot analysis was used to detect alterations in TGF-β1 protein levels, and (b) the concentration of TGF-β1 in the MRC-5 culture supernatant was evaluated by ELISA. Error bars indicate mean ± SEM. **P < 0.01
