Figure 5.

Platelet phagocytosis enhances activation of Akt signaling. (a) Whole-cell lysates from endothelial cells were immunoblotted using antibodies that specifically recognize phosphorylated Akt, Akt, phosphorylated p70 S6K, p70 S6K, phosphorylated ERK1/2, ERK1/2, or β-actin. (b) Densitometric analysis (intensity ratio) for phosphorylated Akt, Akt, phosphorylated p70 S6K, p70 S6K, phosphorylated ERK1/2 and ERK1/2 protein (intensity compared to β-actin). Band intensity of each protein was analyzed using Pro-Plus Image 6.0 quantitative digital imaging system. The experiment was performed thrice