Figure 2.

Hypoxia induced upregulation of LOX expression and increased enzymatic activity in A549 cells. (a) A549 cells were cultured in either hypoxic conditions at 1% O₂ or normoxic condition at 20% O₂ for 18 h. LOX mRNA expression level was determined by RT-PCR and quantitative real-time PCR. (b) Determination of LOX protein level in response to various O₂ condition using western blots; (c) Detection of LOX enzymatic activity by the Amplex Red fluorescence method. Data were from three independent experiments and were presented as mean ± SEM. *p < 0.05, **p < 0.01