Figure 3.

LOX mediates the radioresistance of hypoxic A549 cells. (a) A549 cells were cultured in either hypoxic conditions at 1% O₂ or normoxic conditions with or without βAPN. LOX enzymatic activity was determined by the Amplex Red fluorescence method; (b) Radiation survival curves for A549 cells. Cells were cultured in hypoxic or normoxic conditions for 18 h before radiation at doses of 2, 4, 6 or 8 Gy. 200 μM βAPN was added for the inhibition of LOX enzymatic activity as indicated. Data were from three independent experiments and are presented as mean ± SEM. *p < 0.05, **p < 0.01. (A color version of this figure is available in the online journal.)