Figure 3.

HIF-1α and NOS2 expression in lesion tissue. Three days after beginning of the stress protocol, a full-thickness excisional lesion (1 cm²) was generated on the dorsal skin. Seven days after wounding, lesion was collected and proteins were resolved by SDS-PAGE and immunoblotted for HIF-1α, NOS2, and β-actin. The densitometry of the blots is expressed in arbitrary units to show the HIF-1α/β-actin ratio (a) and the NOS2/β-actin ratio (b). Data are expressed as mean ± SEM (*vs. Control group; #vs. Stress group)

NOS2: inducible nitric oxide synthase; HIF-1α: hypoxia-inducible factor-1α