Figure 9.

The anti-inflammatory effects of sauchinone isolated from SCF4 were mediated by the induction of HO-1. (a) HPLC profile of sauchinone in SCE and SCF4. (b) HO-1 expression increased following treatment with sauchinone. (c) Sauchinone inhibited nitrite production in LPS-stimulated RAW264.7 cells. (d) iNOS expression in LPS-stimulated RAW264.7 cells. (e) Nitrite production in LPS-stimulated cells treated with SCF4 in the presence or absence of ZnPP. (f) iNOS expression in LPS-stimulated cells treated with SCF4 in the presence or not ZnPP. Results of the western blot analysis represent three independent experiments that yielded similar results. Data are the means ± SEM (n = 3), *p < 0.05. HO-1: Heme oxygenase-1; HPLC: high-performance liquid chromatography; iNOS: inducible nitric oxide synthase; LPS: lipopolysaccharide; SCE: S. chinensis extract; SCF4: subfraction 4 of the n-hexane layer of the EtOH extract of the aerial parts of S. chinensis; SEM: standard error of the mean; ZnPP: zinc protoporphyrin