Fig. 6.

Therapeutic regimen of TUDCA attenuates HDM-induced ER stress and AHR. A: Western blot analysis for ER stress markers in whole lung lysates of vehicle-treated or untreated and TUDCA-treated HDM-challenged mice. β-Actin was used as a loading control. B–E: protein bands were subjected to densitometry, and fold change is represented after normalizing to β-actin. *P < 0.05 compared with their respective PBS controls. #P < 0.05 compared with HDM-challenged mice. ‡P < 0.05 compared with mice treated with 0.25 mg/kg TUDCA. F–H: analysis of methacholine-induced AHR in mice. Data are means ± SE of 8–10 mice/group. *P < 0.05 compared with their respective PBS controls. #P < 0.05 compared with vehicle-treated HDM-challenged mice.