Fig. 2.

Effect of PP2 and PP3 on CCK-mediated stimulation of nuclear transcription factors [nuclear factor-κB (NF-κB, A), signal transducer and activator of transcription (STAT)-3 (B), and activator protein (AP-1, C)] in pancreatic acinar cells. Freshly isolated pancreatic acini were preincubated with no additions or with PP2 (10 μM) or PP3 (10 μM) followed by stimulation with 100 nM CCK for 2 h. The nuclear extracts were used for STAT-3, NF-κB, and AP-1 DNA-binding assays that were carried out as described in materials and methods. ns, Not significant. The results are means ± SE from 5 independent experiments.