Fig. 4.

Effect of PP2 and PP3 on CCK-mediated secretion of MIP-1α or regulated on activation, normal T cell expressed and secreted (RANTES) in pancreatic acinar cells. Freshly isolated rat pancreatic acini were preincubated with either 10 μM PP2 or PP3 for 1 h followed by stimulation with 100 nM CCK for 2 h (MIP-1α) or 30 min (RANTES). The supernatant was used to measure MIP-1α (A) and RANTES (B) levels by ELISA as described in materials and methods. Results shown are means ± SE of 4 independent experiments. The basal absolute values for chemokine release in the pancreatic acinar were 73.4 ± 12.9 pg/ml for MIP-1-α and 1.91 ± 0.36 ng/ml for RANTES. *P < 0.05 for CCK, PP2-CCK, or PP3-CCK compared with control, PP2, or PP3 alone, respectively.