Fig. 4.

Immunofluorescence staining of proximal colon shows the normal levels and distribution of NHE-3 in K8^+/− and K8^−/− mice. Proximal colon cryosections from K8^−/−, K8^+/−, and K8^+/+ mice were fixed with 4% paraformaldehyde in PBS for 15 min at room temperature and permeabilized with 0.5% Nonidet P-40 in PBS for 5 min, followed by blocking in 2.5% NGS (normal goat serum) for 120 min at room temperature. There was no difference in NHE-3 staining and apical villin was not altered in K8^+/+ (A), K8^+/− (B), and K8^−/− (C) mice. DAPI was used to stain nuclei. Scale bar 50 μm.