Table 2.

List of primer/probe pairs used for qPCR studies

Gene	Sequence	Company	Reference
β Actin	F primer: 5′ GAT TAC TGC TCT GGC TCC TAG 3′	IDT	Assay no. Mm.PT.39a.22214843.g
	R primer: 5′ GAC TCA TCG TAC TCC TGC TTG 3′
	Probe: 5′ CTG GCC TCA /ZEN/CTG TCC ACC TTC C 3′
GnRH	F primer: 5′ GGG AAA GAG AAA CAC TGA ACA C 3′	IDT	Custom
	R primer: 5′ AGT ACA TTC GAA GTG CTG GG 3′
	Probe: 5′ AGT GGG CAA GGA GGT GGA TCA AAT 3′
GnRH R	F primer: 5′ TCA GCA TTG TCT TTG CAG GA 3′	IDT	Assay no. Mm.PT.45.16240237
	R primer: 5′ TCA CAC ATT GCG AGA AGA CTG 3′
	Probe: 5′ TGA TCT ACC TAG CAG ACG GCT CTG G 3′
RPS29	F primer: 5′ TGA AGG CAA GAT GGG TCA C 3′	IDT	Custom
	R primer: 5′ GCA CAT GTT CAG CCC GTA TT 3′
	Probe: 5′ AGT CAC CCA CGG AAG TTC GG 3′
LHβ	F primer: 5′ CCA GTC TGC ATC ACC TTC AC 3′	IDT	Assay # Mm.PT.45.5612498
	R primer: 5′ GAG GCA CAG GAG GCA AAG 3′
	Probe: 5′ AGT ACT CGG ACC ATG CTA GGA CAG T 3′
FSHβ	F primer: 5′ TTC AGC TTT CCC CAG AAG AG 3′	IDT	Assay no. Mm.PT.45.17694677
	R primer: 5′ TCC AGC ACC AGA ATA AGA TGC 3′
	Probe: 5′ AGC TAC GTC CTG TGC AGT CAG C 3′
CGA	F primer: 5′ AGC ATG ACC AGA ATG ACA GC 3′	IDT	Assay no. Mm.PT.58.31855537
	R primer: 5′ CCT CAG ATC GAC AAT CAC CTG 3′
	Probe: 5′ CCC TCA AAA AGT CCA GAG CTT GCA GA 3′

F, forward; R, reverse.