Table 1.
Primer sets for quantitative PCR of indicated genes, product length (base pairs), and annealing temperature
| Gene | Forward Primer | Reverse Primer | Product Length, bp | TM, °C |
|---|---|---|---|---|
| 18Sr | 5′-GTTCAGCCACCCGAGATTGA-3′ | 5′-CCCATCACGAATGGGGTTCA-3′ | 145 | 60 |
| GAPDH | 5′-TGGAAGGTCTCATGACCACA-3′ | 5′-CAGTGGATGCTGGAATGATG-3′ | 123 | 60 |
| Hif1α | 5′-TTGTGTCAAAGCTGGAGCCA-3′ | 5′-TCAGGCGTTTCTTGCATCTG-3′ | 76 | 60 |
| IGF2 | 5′-CCCGTGGGACGAAATAACAG-3′ | 5′-CCCGTGGGACGAAATAACAG-3′ | 76 | 60 |
| LDHA | 5′-AGGCTTTGCACCCTGAGTTG-3′ | 5′-CTGTCCACCACCTGCTTGTG-3′ | 76 | 60 |
| MYBPC3 | 5′-GTGAAGTGTCCGAGGAAGGG-3′ | 5′-TTGAAAGCCTCTTCCCGTGT-3′ | 76 | 60 |
| MYH6 | 5′-CCACCACATGTTCGTGCTG-3′ | 5′-CCATGCCGAAGTCAATGAACT-3′ | 77 | 60 |
| PDGFB | 5′-TGAGTGCAAGACGCGAACAG-3′ | 5′-CACCACAAAGTTGGCATTGG-3′ | 76 | 58.5 |
| PLN | 5′-TCCTCCACTTTGCTGTTGCC-3′ | 5′-TCAATAGTTGAGGCTCTCCGC-3′ | 76 | 60 |
| RYR2 | 5′-CTCAGGCAGTCCGCCTTTC-3′ | 5′-TCCTGGCAAATTCTGCAAGTG-3′ | 77 | 60 |
| TTN-l | 5′-AGGAGACAGGCCCACACCT-3′ | 5′-CTTCCACCCTCAGCTGTTGAG-3′ | 83 | 58.5 |
| vegfA | 5′-CAATCGAGACCCTGGTGGAC-3′ | 5′-GGGTACACAGGATGGCTTGAA-3′ | 77 | 59 |
TM, annealing temperatue.