Fig. 5.

Impact of biotin supplementation on phosphorylated (p)-mammalian target of rapamycin (mTOR) and p-4E binding protein 1 (4E-BP1) (Ai and Aii), as well as differential gene and protein expression of SLC38A9 (Bi-Biii) and RRADG (Ci-Ciii), in CBK vs. littermate control (CON) hearts. Hearts were isolated from mice at ZT6. Gene expression was assessed through microarray (Bi and Ci) and RT-PCR (Bii and Cii) approaches. Data are shown as mean ± SE for 6 separate observations/group. *P < 0.05 for CON vs. CBK for the same diet. $P < 0.05 for control vs. biotin for the same genotype.