Fig. 5.
Txnip binds to GLUT1 and inhibits cellular glucose uptake. A and B: Txnip-Strep/FLAG (SF)-tag, Txnip C247S mutant-SF-tag, β-arrestin 1 (Arrb1)-SF-tag, arrestin domain containing 1 (Arrdc1)-SF-tag, or empty vector (Empty)-SF-tag (negative control) was coexpressed with GLUT1-HA-tag in HEK-293T cells. Complexes were pulled down with Strep-Tactin resin, subjected to SDS-PAGE, and immunoblotted with anti-HA or anti-SF antibody. C: 2-[3H]deoxyglucose uptake was measured in HEK-293 cells after lentiviral overexpression of arrestin-mCherry fusions. Uptake was normalized to total protein content. 2-Deoxyglucose uptake was significantly inhibited by both Txnip and Txnip C247S mutant but not by other related arrestins. Values are means ± SE. n = 8. **P < 0.01 vs. empty vector.