Fig. 4.

Effect of EP3 receptor deletion on microgliosis after ICH. At 72 h following ICH, immunohistochemical staining for Iba1 was used to evaluate cortical and striatal microglial activation and morphological changes in WT and EP3^−/− mice. A and C: representative high magnification images of coronal brain sections showing the ipsilateral and contralateral cortex (A) and striatum (C) for WT (left) and EP3^−/− (right) mice. Square selections in the insets denote the location of magnified regions. B and D: brown-positive pixel count analysis showed no difference in cortical (B) or striatal (D) microgliosis between WT and EP3^−/− mice. All comparisons include n = 11 WT and n = 11 EP3^−/− mice; ns, not significant.