Fig. 5.

Effect of EP3 receptor deletion on astrogliosis after ICH. At 72 h following ICH, immunohistochemical staining for glial fibrillary acidic protein (GFAP) was used to evaluate cortical and striatal astrocyte activation and morphological changes in WT and EP3^−/− mice. A and C: representative high magnification images of coronal brain sections showing the ipsilateral and contralateral cortex (A) and striatum (C) for WT (left) and EP3^−/− (right) mice. Square selections in the insets denote the location of magnified regions. B and D: brown-positive pixel count analysis showed no significant difference in cortical astrogliosis (B) between WT and EP3^−/− groups, although EP3^−/− mice tended to have increased cortical GFAP immunoreactivity. EP3^−/− mice have significantly increased striatal astrogliosis (D). All comparisons include n = 11 WT and n = 13 EP3^−/− mice; ns, not significant; *P < 0.05.