Fig. 6.

FVIII peptides 721–734 and 729–738, but not 738–750, exhibit reduced hydrogen-deuterium exchange in the presence of antibody F25. The deuterium incorporation level upon F25 binding was identified to be unchanged for cyan areas, reduced more than twice the standard deviation of data for red areas and below twice the standard deviation of data for magenta areas. The inset zooms in on the FVIII A2 domain. Grey areas are uncovered by the peptide map. The obtained peptic peptides are mapped onto a model on the basis of the X-ray crystallographic structure of NovoEight [5]. FVIII, factor VIII.