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. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: J Immunol. 2016 Jun 8;197(2):470–479. doi: 10.4049/jimmunol.1502248

Figure 5. Elevated GM-CSF-neutralizing autoantibodies in aged Rasgrp1-deficient mice.

Figure 5

Concentration of GM-CSF-specific IgG in the serum (a) and BALF (b) of aged control and Rasgrp1-deficient mice measured by ELISA. Symbols represent the mean concentration for individual mice (*p=0.02,***p=0.001, unpaired Student’s t test with Welch’s correction). (c) Concentration of GM-CSF-specific IgG in serum from young Rasgrp1-deficient mice compared to the concentration from aged Rasgrp1-deficient mice in (a) (****p<0.0001, unpaired Student’s t test with Welch’s correction). (d) Inhibition of FDC-P1 cell growth by pooled IgG from aged Rasgrp1-deficient mice. Lines represent the OD450 of FDC-P1 cells grown in the absence of Csf-2 (dashed) or IgG (dotted) in the cell media. Symbols represent the mean values from duplicate wells. (representative of three independent experiments; error bars represent SEM).