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. 2016 Apr 25;25(4):460–469. doi: 10.1111/imb.12233

Figure 2.

Figure 2

Gene copy number and protein expression of suppressor of hairy‐wing [Su(Hw)], modifier of mobile dispersed genetic element 4 (mod[mdg4]) and centrosomal protein of 190 (CP190) in Anopheles stephensi. (A) Southern blot analyses were used to determine the copy number of each gene. Genomic DNA samples were digested with EcoRI and hybridized with Su(Hw)‐, mod(mdg4)‐ and CP190‐specific probes. Approximate fragment lengths in kb are indicated to the left of each image. (B) Immunoblot analyses of ovaries 48 h post‐bloodmeal extracts were used to determine Su(Hw), Mod(mdg4) and CP190 protein expression. Proteins were detected with Drosophila polyclonal anti‐Su(Hw), anti‐Mod and anti‐CP190 antibodies. Approximate weights in kDa are indicated to the left of the image.