(A) LDPI images of mice after the ischemic hindlimb surgery. Ischemia was introduced by ligating the femoral artery in the right leg. The red color intensity represents the relative intensity of perfusion. The ischemic leg imaged after 24 h (A-1) was treated for 28 days with 2.5 μg of VEGF in a fibrin gel (A-2), 2.5 μg of VEGF + unmodified alginate gel in a fibrin gel (A-3), and 2.5 μg of VEGF + alginate sulfates in a fibrin gel (A-4). (B) Quantification of the mean perfusion in the legs of mice with ischemic hindlimbs. The ischemic leg was treated with 2.5 μg of VEGF in a fibrin gel (▲), fibrin gel + alginate (●), and fibrin gel + alginate sulfates (■). The perfusion in the ischemic limb was normalized to the perfusion in the nonischemic limb (n = 4 mice, p < 0.05).