Figure 2. Pbx1-d overexpression impaired pTreg cell induction.

bx1-d mRNA expression in Foxp3⁺ and Foxp3⁻CD4⁺ T cells purified from B6.Foxp3^egfp, Pbx1-d Tg.Foxp3^egfp, and B6.Sle1a1.Foxp3^egfp mice analyzed by conventional (A) and quantitative (B) RT-PCR with the data presented relative to B6 (RQ; n = 3). C. Frequency of Foxp3⁺ Treg cells in the mLN of 10–12 month-old B6, Pbx1-d Tg, and B6.Sle1a1 mice. Each symbol represents a mouse. D. Foxp3 induction in CD4⁺CD25⁻T cells in the presence of TGF-β, presented as the ratio of induction with over without RA. E–H. Mixed-BM chimera analysis of Treg cells. E. Experimental design. F. Gates for Foxp3⁺Nrp-1⁺ tTreg and Foxp3⁺Nrp-1⁻pTreg cells within the total CD4⁺CD8⁻CD45.1⁺ or CD45.2⁺ thymocytes. Percentage of pTreg (G) and tTreg cells (H) in the chimeras with each linked symbol representing a mouse. *P < 0.05, ** P < 0.01.