Figure 3. Pbx1-d Tg expression in CD4⁺ T cells expanded GC lymphocytes.

Absolute numbers of lymphocytes (A), percentages (B) and absolute numbers (C) of B220⁺GL-7⁺FAS⁺ GC B cells in the spleen and mLN from 2 month-old B6, Pbx1-d Tg, and B6.Sle1a1 mice. D. Representative FACS plots for CD4⁺CXCR5⁺PD-1⁺Bcl6⁺Foxp3⁺ T_FR and CD4⁺CXCR5⁺PD-1 ⁺Bcl6⁺Foxp3⁻T_FH cells. Percentages (E) and absolute numbers (F) of T_FH and T_FR cells. n = 5–10. G–I: Follicular T cell expansion in response to NP-KLH immunization in mixed BM chimeras. G: Experimental strategy. Absolute numbers (H) and percentages (I) of T_FH and T_FR cells 7 d after immunization gated according to their strain of origin: CD45.1: B6, CD45.2: Pbx1-d Tg. Each symbol (linked in I) represents a mouse. *P < 0.05, **P < 0.01.