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. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: J Immunol. 2016 Jun 13;197(2):458–469. doi: 10.4049/jimmunol.1502283

Figure 5. Pbx1-d intrinsically regulated TFH cell differentiation and amplified recall responses.

Figure 5

A. Experimental design for primary and recall response after mixed adoptive transfer of CD90.1+ B6.OT-II and CD90.2+ Pbx1-d Tg.OT-II or Sle1a1.OT-II CD4+ T cells into (B6 x B6.Thy1a)F1 mice. Percentages of CD90.1+ B6.OT-II and CD90.2+ Pbx1-d Tg.OT-II or Sle1a1.OT-II CD4+ T cells (B and D) and TFH cells (C and E) from each genotype in the primary (B and C) and recall (D and E) responses. F. Fold increase of TFH cells in recall vs. primary response, calculated as the ratio of CD90.2+ /CD90.1+ TFH cells. Representative flow cytometric plots and corresponding percentages of CD4+CD69+ activated T (G and H) and TEM / TN CD4+ T cells ratios (I and J) in CD90.1+ B6.OT-II and CD90.2+ Pbx1-d.OT-II Tg donor cells in the recall response. Each linked symbol represents a recipient mouse. n = 5–6, *P < 0.05, **P < 0.01, ***P < 0.001.