Figure 8. Human PBX1 binds to the promoter of miR-10a, miR-21, and miR-155.
A. miRNA luciferase constructs containing predicted PBX1 binding sites were selected using Jaspar3. The regions used in the luciferase assays are indicated for each locus. B. Dual luciferase analyses of miR-10a, miR-21 and miR-155 expression in the presence of either PBX1-b or PBX1-d, showing fold change relative to the absence of PBX1 expression plasmid. All results are expressed as mean ± SD from at least three independent experiments.
