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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Adv Drug Deliv Rev. 2016 Mar 21;103:174–186. doi: 10.1016/j.addr.2016.03.005

Fig 1. si/shRNAs use the endogenous miRNA machinery for gene silencing.

Fig 1

While shRNAs mimic pre-miRNAs, siRNAs resemble mature miRNAs. Like pre-miRNAs, shRNAs are produced in the nucleus and are exported to the cytoplasm, where they are further processed by Dicer into mature siRNAs. Synthetic siRNAs, upon transfection, are loaded directly onto the RISC in the cytoplasm.