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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Adv Drug Deliv Rev. 2016 Mar 21;103:174–186. doi: 10.1016/j.addr.2016.03.005

Fig 3. Design of AgoshRNA.

Fig 3

Conventional shRNA is designed to mimic conventional pre-miRNAs like miR-150 shown in the left panel. In contrast AgoshRNA is designed to mimic the pre-miR-451 as shown in the right panel. Conventional shRNA typically has a stem length of 19–22 nt and a larger loop (~10 nt) compared to AgoshRNA which has a 16–18 nt stem and a smaller (4 nt) loop. Whereas the conventional shRNA is processed by dicer and any of the 2 stem strands can give rise to guide strand of siRNA, AgoshRNA is processed by Ago2 and only the top strand along with the loop is loaded onto RISC (because of Ago2-mediated cleavage of AgoshRNA). Mature miRNA sequence is marked in red.