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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Adv Drug Deliv Rev. 2016 Mar 21;103:174–186. doi: 10.1016/j.addr.2016.03.005

Fig 4. Multiplexed shRNA.

Fig 4

Multiple shRNAs can be expressed under control of different Pol III promoters (top). Multiplexed shRNAmiRs can be expressed in the context of polycistronic endogenous miRNA cluster (middle) or as multiple pri-miRNAs, each containing the minimal flanking sequence derived from different endogenous miRNAs (bottom). While the maximum number of shRNAs is limited to 4 in the polycistronic context, the number is virtually unlimited using different miRNA flanks.