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. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: J Immunol. 2016 Jun 6;197(2):565–579. doi: 10.4049/jimmunol.1600342

FIGURE 5.

FIGURE 5

H. capsulatum induces nuclear HIF-1α in Mϕs. A and B, mRNA expression of HIF-1α, VEGF, and PGK-1 was quantified by qRT-PCR relative to uninfected controls. A, GFP+ monocytes were collected at days 1, 3, and 7 following intranasal infection with GFP expressing yeast. (n=12 mice/group; representative of 3 experiments) B, BMDMϕs were collected 24 h post-infection. (n=8 experiments/group) C, Immunoblot of HIF-1α and β-actin from whole-cell lysates of Mϕs infected with H. capsulatum for 24 h. (representative experiment of 3) D, Confocal images of BMDMϕs uninfected or infected for 24 h followed by nuclear staining (DAPI) and staining for HIF-1α. (representative experiment of 3) *p<0.05; **p<0.01; ***p<0.001