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. Author manuscript; available in PMC: 2017 Jul 15.
Published in final edited form as: J Immunol. 2016 Jun 6;197(2):565–579. doi: 10.4049/jimmunol.1600342

FIGURE 6.

FIGURE 6

In the absence of HIF-1α, the transcription factor CREB drives elevated IL-10 production in response to H. capsulatum infection. A, BMDMϕs were infected for 48 h then lysed to determine fungal burden (n=4–6 mice/group; representative of 3 experiments) B, D, F IL-10 concentration was determined in the cell culture supernatants by Magpix. C, E and G, mRNA expression of IL-10 was quantified by qRT-PCR relative to uninfected controls. B, BMDMϕs were infected for 48 h. (n=6 mice/group; representative of 3 experiments) C, BMDMϕs were infected for 8, 24, or 48 h. (n=6–9 mice/group; representative of 5 experiments) D, Lyz2cre and Lyz2cre Hif1αfl/fl, and Lyz2cre Hif1αfl/fl Hif2αfl/fl double knockout BMDMϕs were infected for 24 h. (n=6 mice/group; representative of 3 experiments) E, F, BMDMϕs were infected for 48 h after treatment with DMOG (control), a HIF-1α-CBP interaction inhibitor (chetomin), or a CREB-CBP interaction inhibitor (KG-501). (n=9 mice/group; representative of 3 experiments). Treatment groups were compared to the DMOG control. F, BMDMϕs were infected for 48 h after treatment with scrambled siRNA (control) or CREB siRNA. (n=6 mice/group; representative of 3 experiments) *p<0.05; **p<0.01; ***p<0.001