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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Dev Neurobiol. 2016 Jan 25;76(10):1092–1110. doi: 10.1002/dneu.22377

Figure 5.

Figure 5

Drebrin promotes the targeting of microtubule plus tips into axonal filopodia which is not required for drebrin-mediated stabilization of filopodia. (A) Example of GFP-EB3 imaging in an RFP-drebrin E1 expressing neuron. The leftmost panel shows the distal axons and the boxed in region denotes the subsequent panels reflecting stills from the timelapse (time shown in seconds in panels). An EB3 comet enters the filopodium at 10 sec and extends to the tip of the filopodium by 50 sec. (B) Analysis of the duration of axonal filopodia pre and post a 30 min treatment with 3 nM vinblastine, which as described in the text minimizes the entry of EB3 comets into filopodia in both RFP and RFP-drebrin E1 expressing neurons. The data are normalized to the pre-treatment condition for both groups (RFP and RFP-drebrin E1 expressing neurons; n=7 and 12 respectively). Vinblastine treatment did not affect the duration of filopodia in either group.