Figure 2.

Concentration‐dependent inhibition of hippocampal glutamate release by Δ⁹‐tetrahydrocannabinol (THC). (a) Time course of the inhibition of the initial slope of fEPSPs (indicated by shaded box in upper traces) by Δ⁹‐THC across concentrations, applied at the time indicated by the horizontal bar. (b) Upper, representative averaged traces (n = 5–7 sweeps) demonstrating the effect of Δ⁹‐THC and reversal of its effects by the neutral CB1 antagonist PIMSR1. Lower, summary time course of recordings (n = 7 recordings) demonstrating the reversal of Δ⁹‐THC effects by PIMSR1. (c) Concentration–response curve for Δ⁹‐THC (n = 6–7 slices per concentration). The EC₅₀ (solid line) was calculated to be 707 nM. Dashed lines indicate the 95% confidence interval. [Colour figure can be viewed at http://wileyonlinelibrary.com]