Table 1.

Sequences of Theileria parva gene primers incorporated into fusion primers, primer concentrations and annealing temperatures used to generate PCR amplicons from antigen genes for high throughput sequencing.

Gene	Primer sequences (5′–3′)	Concentration (pmol/reaction)	Annealing temp. (°C)	Amplicon sizea
Tp1	for-CTGGTGTACAATTTGGTGGG	20	55	428
rev-AACTTNMCTTCTTGCGAACC	20
Tp2	for-ATGAAATTGGCCGCCAGATTA	10	49	492
for-GCCAGATTAATHAGYCTTTAC	10
rev-AGATTTGTCACTAYCTGTWBYAGG	25
rev-AGATTCGTCCTCAYCTGTWBYAGG	25
Tp3	for-AGCAGATTTCACTCAAGCTGC	20	54	407
rev-TCCCCCAGAACATTAAACGG	20
Tp4	for-GCAACACAATACTTTGCAGG	10	54	424
rev-CCTCAAACACWCCACAAGTTCC	10
Tp5	for-GTATGCTCGGTAATGGCAG	10	55	347
rev-GATTTTGGTCGCTTCAGGC	10
Tp6	for-CGTCCAATAATTTACGATGTGAG	10
rev-GCTTAAGTGGGTTAAGGAGACA	10	55	326
Tp7	for-TGAAGAAGGACGACTCGCAC	20	58	292
rev-TCCTCGTCAGTGACGTCGG	20
Tp8	for-ATCCACAACCAAGTGCCCAG	10	54	305
rev-TGCTATTGCGAGTCAACAG	10
Tp10	for-GGTCGTCTGACAATAACC	10	49	314
rev-CTAMCATGTAAATCCAGC	20

^aThe expected amplicon sizes (excluding the adaptor and Multiplex Identifier (MID) sequence of the fusion primer), based on the Muguga reference genome sequence.